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发表于 2017-12-04 00:00

题目:Unraveling Physico-Chemical Properties of the Nuclear Organelles by Noninvasive Optical Tools

时间:2017年12月04日(周一) 下午3:30pm


报告人:Dr. Artem Pliss, Research Associate Professor, University at Buffalo, State University of New York


Abstract. Interactions between soluble proteins in the cell nucleus lead to formation of discrete spherical droplets, known as nuclear organelles, which are vital for cellular regulation. However, the physico-chemical properties of the nuclear organelles are still poorly understood, because they are highly challenging to study in a live cell environment, without disruption of their biological functions. Recently, developments of two complimentary optical techniques, - Raman spectroscopy and Fluorescence Lifetime Imaging (FLIM), made possible to investigate the formation, and maintenance the nuclear organelles. These techniques have unraveled that the molecular content of the nuclear organelles is dynamically changed during the cell development and can also serve as a marker of abnormal cellular states. This presentation will focus on the recent developments of Raman Spectroscopy and FLIM techniques for studies of the cellular function.



Dr. Artem Pliss currently holds a position as Research Associate Professor in the University at Buffalo, State University of New York. He obtained his B.S. and M.S. in Biology majoring in Cell Biology and Histology, Kiev State University, Kiev, Ukraine. He received his PhD in Cell Biology and Pathology from Charles University, Prague, Czech Republic. After postdoctoral training in the Department of Biological Sciences, SUNY at Buffalo, he joined the Institute for Lasers, Photonics and Biophotonics in the University at Buffalo. Dr. Pliss is specialized in bioimaging including conventional, confocal and multiphoton microscopy, fluorescence lifetime imaging, second harmonic microscopy, label-free vibrational spectroscopic imaging, and transmission electron microscopy. He has co-authored over 40 peer-reviewed articles including Nature Photonics, PNAS, JACS, Biomaterials and etc.

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